Molecular Surveyor dhfr-dhps - Methodology

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Data provided by Inbarani Naidoo and Cally Roper at the London School of Hygiene and Tropical Medicine
Literature searches used Pubmed, African Journal Online and Bioline
Search terms were "malaria" and "dhfr" or "dhps" or "540" or "437" or "436" or "581" or "51" or "59" or "108" or "164"
Studies had to include analysis of dhfr or dhps genes in P falciparum isolates sampled from infected people living in a clearly identified geographical site(s) in Africa
Abstracts and full text of suitable studies were reviewed and prevalence of wild-type and mutant sequences was recorded
Searches conducted from October 2005 to January 2011

Data provided by Mallika Imwong and team at the Faculty of Tropical Medicine, Mahidol University
Literature searches used Pubmed
Searches terms were "falciparum" and "Asia" and "dhfr" or "dhps"
Studies had to include analysis of dhfr or dhps genes in P falciparum isolates sampled from infected people living in clearly identified geographical site(s) in Asia
Abstracts and full text of suitable studies were reviewed and prevalence of wild-type and mutant sequences was recorded in a total of 33 studies
Searches conducted in March 2010

Data provided by Vladimir Corredor, Faculty of Medicine, Universidad Nacional de Colombia
Literature searches used Pubmed, SciELO, Bioonline and Biblioteca Virtual de Salud
Search terms were "malaria drug resistance" or "dhfr falciparum" or "dhps falciparum" and "South America" or "[name of each country comprising South America]"
Abstracts and full text of suitable studies were reviewed and prevalence of wild-type and mutant sequences was recorded in a total of 24 studies
Search conducted in 2009 and updated in Feb 2011

Study locations and years are based on information presented in publications or provided by authors
‘Estimated location’ is used when specific site names could not be found or the publication merged samples from multiple sites
Sample collection years that were estimated by the study authors are indicated as such
Studies for which a sample collection time could not be found were attributed an estimated sample collection time of 3 years prior to the year of publication.