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Procedures

WWARN is continually updating a library of standardised procedures that offer guidance in the execution of various activities in the fields of clinical, in vitro, pharmacology, data collection and molecular analysis for antimalarial drug resistance researchers. Please read our tools and resources usage guidance.

For resources that relate to other infectious and emerging diseases, visit iddo.org.

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EXT05
msp1 PCR
PCR amplification of the polymorphic marker msp1 to distinguish recrudescences from reinfections
Version: 1.0 Author: Mekong Molecular Surveillance Network
EXT03
DNA Extraction by QIAamp DNA Mini Kit
Extracting Plasmodium spp. DNA from dried blood spots by QIAamp DNA Mini Kit
Version: 1.0 Author: Mekong Molecular Surveillance Network
EXT02
DNA extraction by Chelex
Extracting Plasmodium spp. DNA from dried blood spots by Chelex 100
Version: 1.0 Author: Mekong Molecular Surveillance Network
EXT01
Collection of blood on filter paper
Molecular Testing for Malaria - preparing dried blood spots by finger prick
Version: 1.0 Author: Mekong Molecular Surveillance Network
INV08
Estimation of Plasmodium falciparum drug susceptibility by the SYBR® Green assay
Drug susceptibility assay measuring SYBR®Green I fluorescence in laboratory strains and field isolates
Version: 1.0 Author: WWARN
INV09
Estimation of Plasmodium falciparum drug susceptibility ex vivo by HRP2 ELISA
Drug susceptibility assay measuring histidine-rich protein production by ELISA in fresh isolates
Version: v1.0 Author: WWARN
CL10
Preparing malaria blood smears
Preparing thick and thin blood smears with Giemsa staining
Version: 2.0 Author: WWARN
INV07
Estimation of Plasmodium falciparum drug susceptibility by the 3H-hypoxanthine uptake inhibition assay
Drug susceptibility assay measuring radioactive hypoxanthine uptake in laboratory strains and fresh isolates.
Version: v1.0 Author: WWARN
INV03
Preparation of drug plates for susceptibility assays
Preparation of drug dilutions in dry or fresh plates for drug susceptibility assays
Version: v1.2 Author: WWARN
MOL05
Copy number estimation of the Plasmodium falciparum pfmdr1 gene
Estimating pfmdr1 gene copy number by real-time PCR
Version: v1.1 Author: WWARN

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